Significant attention has centered upon metal complexes capable of binding. Design, spectral characterization and biological evaluation. Synthesis, characterization, dna binding interaction studies and antimicrobial screening mixedligand cu ii and mn ii complexes omar h. Synthesis, characterisation and dna binding studies of. Intermolecular noes place the metal complex in the major groove of the oligonucleotide. Investigation of dna binding mechanism, photoinduced cleavage. Metal n 2 o interactions in the gas phase or inert matrices. Dna and bsabinding studies and anticancer activity against. In the recent years, the interaction between transition metal complexes with dna and proteins have been intensively studied.
New functionalized ligands for luminescent metal complexes. The synthesis of luminescent metal complexes is a very challenging task since they can be regarded as the starting point for a lot of different areas. Pdf experimental techniques employed in the study of metal. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a duplex dna have been determined. Studies on dna binding to metal complexes of sal2trien article in biochemistry moscow 721. Design, syntheses and functional studies of dna binding metal. An overview is presented of the key role for heavy metal compounds in medicine with a focus on dna binding, and on pt compounds used in treatment of cancer. Binding of synthesized complexes with calf thymus dna ct dna was studied by spectroscopic methods and. An external file that holds a picture, illustration, etc. In this study, we characterized nine natural xchh, cxhh, ccxh and cchx zfs in terms of their znii and coii binding properties, such as complex stoichiometry, spectroscopic properties and metaltopeptide affinity.
Transition metal complexes play an important role in nucleic acids chemistry for their. Synthesis and characterization of ruthenium metal complexes. The dna cleavage studies of metal complexes showed more prominent activity in the presence of h 2 o 2 compared to that in the other metal complexes. Crystal field theory versus molecular orbital theory. Detailed dna binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Morphological studies were carried out by scanning electron microscopic method. Synthesis, characterization, dna binding interaction studies. For example, square planar complexes penetrate deeper into the dna base pairs than octahedral or tetrahedral complexes do. An introduction to transition metal chemistry a transition metal is an element that has a partly d or f subshell in any of its common oxidation states. Prepared complexes showed enhanced dna binding tendency than the parent drug that might be of interest for future research. Elemental analysis table 1 shows that the ligands and the metal salts are in the ratio of 2. Metallointercalators with different metal centers, oxidation states, coordination geometries, and overall sizes will exhibit varying depths of insertion. Synthesis, characterization and antimicrobial screening. Over 10 million scientific documents at your fingertips.
Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy. The path for metal complexes to a dna target ncbi nih. Synthesis chracterization and dna binding studies of novel. Studies on dna binding to metal complexes of sal2trien. All metal complexes showed significant nuclease activity in the presence of h 2 o 2. Jan 26, 2010 recent advances in our understanding of the reaction pathways for n 2 o reduction by metal ions in the gas phase and in heterogeneous, homogeneous, and biological catalytic systems have provided provocative ideas about the structure and properties of metal n 2 o adducts and derived intermediates. Dna binding, in silico docking and in vitro biological screening of some transition metal complexes of schiff base ligand as potential blockers of cancer causing receptors, int. Metal complexes as structureselective binding agents for.
Therefore, viscosity measurements were carried out to further clarify the interaction of metal complexes and dna. Dna binding, amelioration of oxidative stress, and molecular docking study of znii metal complex of a new schiff base ligand. Transitionmetal complexes that bind and cleave dna elizabeth wilkinson literature seminar april 3, 1990 dna is a remarkably complex molecule. Pdf dna binding, amelioration of oxidative stress, and. Metal binding properties of zinc fingers with a naturally. The interactions between the metal complexes and dna were studied using electronic absorption method. Studies on heat resistant metal composite of nsubstituted maleimide jyotichaudhary, swati purohit and sumanjinger department of polymer science, m. From the spectral studies an octahedral geometry was proposed for the copper complex. Dna binding studies of metal complexes mn ii, co ii, ni ii, cu ii to dna helix has been characterized through absorption spectral titrations, significant hypochromism with a red shift of 10 nm bathochromism of absorption band implicates intercalative mode of binding and is likely that the all complexes with aromatic chromophore. There is evidence that the imidazole ring is an important binding site for metal ions in serum. We revealed that xchh and cxhh zfs form ml complexes that are 45 orders of magnitude weaker in comparison to cchh zfs. Electronic supporting information files are available without a subscription to acs web editions.
The low molar conductance values of the complexes reveal. Selective binding of transition metal complexes to. Metallointercalation is a dna binding mode that has been extensively studied. Molecular modelling studies were also performed to confirm the geometries of the complexes. Pdf the present paper is an overlook on metal complexesdna interactions, their classification and the experimental techniques. The formation of polynuclear metal complexes was only considered highlighting the key role played by the phenoxide oxygen atom in binding two metal centres in a bridge disposition. Furthurdna binding studies were carriedout for the complexes. Coordination compounds are formed between a metal atom or newlineion and a molecule. Synthesis, characterisation and dna binding studies of metal. Synthesis, characterization and dna binding study of mixed.
The ligand and their metal complexes were screened for their antimicrobial activity against bacteria. These results are important for future studies on ligand binding to triple helices as well as on triple helix formation studies using intercalators appended to the third strand. Transition metal complexes can bind to dna through covalent bonding via replacement of a labile ligand of the complex by a nitrogen base of dna such as guanine n7 or noncovalent interactions such as intercalation, electrostatic, or groove binding. Find, read and cite all the research you need on researchgate.
This arrangement allows two metal ions to stay close to each other and consequently these. Binding studies of metalsalphen and metalbipyridine. Alobaidi chemistry department, education college for women, alanbar university abstract. Josephs college autonomous, tiruchirappalli620002, tamil nadu. Synthesis, electronic structure, dna and protein binding, dna. Their first application was reported by wang and coworkers in 2008 12, who developed a polystyrene.
The biological and binding properties of the complex with dna have been investigated by uv spectroscopy and thermal denaturation experiments. Pdf synthesis, characterization, dna binding properties. Also, the shift in carbonyl frequency on binding to the metal centre is observed in both the complexes. Binding and activation of n2o at transition metal centers. Voltammetric studies of the interaction of transitionmetal complexes with dna. Optical photophysical techniques are widely used to study the binding model of the ligand, metal complexes, and dna but not to give sufficient clues to support a binding model. It is found in three main dou ble helical forms, the righthandeda form and b form the watsoncrick double helix, and the left handed zform lj. Mar 30, 2010 synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy benzoyl hydrazone.
Recent studies have shown that a variety of transition metal complexes have significant potential as probes for sequence and structurespecific dna binding 2. Structural data of dna binding and molecular docking. The formation of metal and oxygen bond mo can be seen at 530cm1 and 653cm1 for copper and cobalt complexes respectively. Synthesis, characterisation and dna binding studies of metal complexes of norfloxacin s. Jayabalakrishnan2 1department of chemistry, kumaraguru college of technology, coimbatore, tn, india 2department of chemistry, sri ramakrishna mission vidyalaya college of arts and science, coimbatore,tn, india. Due to cationic character, threedimensional structural profiles, and propensity for performing hydrolysis, redox, or photoreactions of metal ions and complexes, have a natural ability for interacting with dna. The chemicals include 4hydroxybenzaldehyde, urea, acetyl acetone, magnesium bromide, 4aminoantipyrine, dimethylsulphoxide transition metal chlorides. Dna and bsabinding studies and anticancer activity. The dna cleavage studies of metal complexes presented more protruding activity in the. Dec 30, 2014 the binding constants kb of the complexes were determined as 2. Synthesis and dna binding studies of niii, coii, cuii and znii metal complexes of n1,n5bispyridine2methylenethiocarbohydrazone schiffbase ligand. In general, mercuryi1 has the greatest binding power for nitrogen ligands of any of the metal ions. Binding studies of metalsalphen and metalbipyridine complexes.
Synthesis, characterization, dna binding properties. Metal complexes have great diversity in size and structure, as well as useful photophysical and electrochemical properties in many cases, and consequently they have become an important class of structureselective binding agents for nucleic acids. Here we describe recent results on a variety of transition metal complexes targeted to dna to illustrate. Synthesis, crystal structure, and dnabinding studies of a. Final report of work done on the minor research project. A transition metal is an element that has a partly d or f. More specific subject area, dna binding, antioxidant, molecular docking. Aug 12, 2014 synthesis and characterization of some transition metal complexes with nphenyln substituted phenyl thiourea. Structure and reactivity of metal complexes bound to dna. Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy benzoyl hydrazone.
Synthesis, characterization and dna binding study of. Absorption titration experiments were performed by keeping fixed concentrations of the metal complexes while gradually increasing the concentration of ct dna. Synthesis, characterization, antimicrobial, dna binding and. The transition metal complexes may be used as potential anticancer drugs, because they bind to calf thymus dna via an intercalation binding mode with the binding consts. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a. Furthermore, the binding of ethidium is stronger to the triplex than to the polyda. Luminescent complexes, in fact, can be used for technological, industrial, medical and biological applications. Structural data of dna binding and molecular docking studies. Competitive binding studies with ethidium bromide have been performed by fluorescence spectroscopy in order to investigate ability intercalation of the znii complex to dna. Sukhadia university, udaipur 301, rajasthan india purohit.
Studies on heat resistant metal composite of nsubstituted. In the case of metals metal aqua complexes and other metal complexes, the subject of their interaction with dna is relevant for a number of reasons. Design, syntheses and functional studies of dna binding metal complexes final seminar april 10, 1998 antitumor activity of many anticancer drugs is based on their interaction with dna five basic interaction types are observed. Tuned structure and dna binding properties of metal complexes. Synthesis and characterization of some transition metal. Synthesis, dna binding, and antimicrobial studies of novel metal. The transition elements are taken to be those of groups 312, plus the lanthanides and actinides. The dna binding ability of inert chiral transition metal complexes has attracted considerable interest. The dna binding and protein binding studies suggest that the complex 3 exhibits more effective binding activity kb5. These systems range from in vivo aspect such as the endogenous presence in the nucleus of metal ions, the role of zinc in rna polymerase, the mutagenesis and eventual carcinogenesis of. Dna binding studies were carried out by viscosity method. Dna binding studies were performed on h2l and its three complexes.
Many studies of the reactions of n 2 o with metal atoms or ions in the gas phase or in inert matrices have been performed with the goal of obtaining fundamental thermodynamic, kinetic, and mechanistic information potentially relevant to more complex systems. Thus through a series of experiments in this thesis, attempts have been made to correlate the structure and dynamics of metal complexes bound to dna. The results showed that the metal complexes are biologically active. Design, syntheses and functional studies of dna binding. In modern medicinal chemistry, transition metal complexes for their binding properties has become suitable candidates as anticancer and antitumor drugs, the most important examples are cisplatin, carboplatin, and oxaliplatin. Tlc was also used to study the interaction of the 9etg with the metal complexes. Structural data of dna binding and molecular docking studies of.
The necessity for cellular regulation of dna led to the development of metallonucleases to catalyze and repair dna strand breaks. Synthesis, characterization and dna binding study of ruthentumii thiosemicarbazone complexes k. Metal complex dna interactions provides a comprehensive overview of this increasingly diverse field, presenting recent developments and the latest research with particular emphasis on metalbased drugs and metal ion toxicity. In general, transition metal complexes act as dna binding agents, and. Request pdf on may 1, 2014, poonam inamdar and others published synthesis, characterization and dna binding study of mixed ligand based metal complexes find, read and cite all the research you. Design, spectral characterization and biological evaluation of unsymmetrical 11membered tetraaza macrocyclic complexes containing thiourea stella shalini a.
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